The thioglycolate test had growth throughout the agar with thicker growth at the surface, which revealed that the microbe’s oxygen class was facultative aerobe. The results did not meet these thresholds, and this is the reason that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected. BaseSpace has many apps that provide different information about the genomic data. Retrieved from List of prokaryotic names with standing nomenclature database. Cells of strain AJ003T were aerobic, Gram-stain-positive, cocci-shaped and orange. BLAST provided many species level classifications with similar query cover and percent identity. The cells inoculated onto Eosin Methylene Blue (EMB) and MacConkey Agar (MAC) plates. 16S rRNA gene sequence similarity of strain SCU63 T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %. They are prokaryotic in nature meaning they are the earliest forms of the cell without a nucleus.. Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. The bacterium is Gram-positive and possesses all the characteristics of the genus Planococcus. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. EMB is selective for gam-negative bacterium, if the microbe is gram negative and can ferment the sugars present then the plate experiences a color change. the following information is not yet verified. By Ethan Hanson Classification: Kingdom: Bacteria Phylum: Firmicutes Class: Bacilli Order: Bacillaes Family:Planococcaceae Genus: Planococcus Species: Planococcus halocryophilus Planococcus halocryophilus is an aerobic, gram-positive bacterium that is found in arctic permafrost. Micrococcus varians The analysis of the DNA with BaseSpace revealed unreliable data. I was unable to grow any microbes from the wood stove but the growth from the previously frozen wood was abundant. The Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and 3739 CDS. Pigments may be produced by some species; ranging from cream, pale yellow to light pink. Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia.It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest.It is associated with citrus, but it attacks a … The genus Planococcus was originally proposed by Migula [1] to accommodate Gram-stain-positive, aerobic and motile cocci of the family Planococcaceae in the phylum Firmicutes. The APP provides an extensive list of regions that were specifically identified as the above elements. PAMC21323 is a gram (+), motile, psy-chrotrophic bacteria, which can grow over a broad temperature range (5–40 °C). Image 5: Krona Classification Chart from BaseSpace displays the large portion of unclassified reads. The catalase test produced bubbles and the oxidase test produced a blue color change on the strip indicating that the microbe was positive in both tests. The API 20e tests had negative results for all for the 21 tests: Image 3. Gemella
Image 5 and 6 are from the Kraken app. compare with Marinococcus, Migula W. (1894) Uber
Gram stain. radioresistant cocci called Deinococcus
Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. Inst. The genus Staphylococcus is found in section 12, volume 2 of Bergey's Manual of Systematic Bacteriology: the Gram-Positive Cocci.The section includes aerobic genera (Micrococcus, Planococcus and Deinococcus), facultatively anaerobic genera (Staphylococcus, Stomatococcus, Streptococcus, Leuconostoc, Pediococcus, Aerococcus and Gemella) and anaerobic genera (Peptococcus, … The percent of contigs read needed to be greater than 80% and the percent of those reads that needed to agree upon a classification to trust that classification to the genus level was 80% and to the genus level was 60%. When the colonies grew one colony was transferred onto fresh TSA plates. Decomposing communities frequently have a mix of fungi and bacteria. Phylogenetic and signature nucleotides analyses revealed that strain Y42T belongs to the genus Planococcus. The gram stain was performed to assess the thickness of the peptidoglycan wall. to the Genus. nov. M. Engelhardt , K. Daly , R. Swannell , I. These tests reveal nothing more than a conformation that it is indeed a gram-positive microbe. Verified Small gram negative microbes these results are confirmation that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was.! The morphological and physiological analyses made on the TSA agar thickness of the morphological and physiological analyses on... Mucilaginsosus Micrococcaceae are not a coherent group 98.6 % carotenoid-producing species isolated from beach... Tests reveal nothing more than a conformation that it is natural for bacteria these! Similar environment piece of wood is designed for enteric gram negative coccobacilli with O-shaped morphology characteristics! P., & Parte, A. C. 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