desmoglein 1 antibody

Cat. Our Desmoglein-1 Antibodies can be used in a variety of model species: Bovine, Human. products description : Mouse monoclonal Desmoglein 1 antibody. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Get the latest news, product updates, and promotions: Detects human Desmoglein-1 in direct ELISAs and Western blots. Desmoglein Antibodies (1 and 3) Test Resources. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Author information: (1)St John's Institute of Dermatology, St Thomas' Hospital, London, UK. of experiments: ≤ 5000 (IHC, 50 µl/slide) Delivery Time: usually 1-7 working days . Desmoglein‑1 in Human Skin Hyperplasia. ncbi mol weight : … In humans, this protein is encoded by the gene DSG1. Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. These desmoglein gene family members are located in a cluster on chromosome 18. Antibody: Rat Desmoglein-1 (DSG1) Polyclonal Antibody, Application: Validated by immunofluorescence labeling (1:100). Desmoglein-1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. Not for use in clinical diagnostics. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. The protein may also be known as DG1, DSG, PPKS1, CDHF4, EPKHE, and cadherin family member 4. The severity of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels. Immunogen: Desmoglein 1 antibody was raised in mouse using recombinant human polypeptide desmoglein 1, extracellular part EII, EIII and EIV as the immunogen. Recombinant Human Desmoglein‑1 Fc Chimera (Catalog #. Immunogen: Synthetic peptide (15-aa) derived from the C-terminal region of human Desmoglein-1 protein, Species homology of immunogen: Synthetic peptide sequence shows 93.3% homology to mouse or rat sequence, Storage buffer: PBS, pH 7.2, 0.1% sodium azide. The monoclonal anti-desmoglein 1 antibody stains basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. Human Desmoglein-1 is a type I transmembrane glycoprotein of 1049 amino acid (aa) residues with a 23 aa signal peptide and a 26 aa propeptide. Desmoglein-1 (DSG1) is a transmembrane glycoprotein belonging to the family of cadherins. Design: Retrospective study. uniprot acc num : Q02413. : 651111 Quantity: 5 mL No. 2001 Apr;144(4):775-80. pemphigus and differential diagnosis of pemphigoid, EBA etc.MethodELISA with recombinant antigens desmoglein 1 and 3. Desmoglein‑1 in Human Skin Hyperplasia. Please Note: Optimal dilutions should be determined by each laboratory for each application. Filter your results: No product specific FAQs exist for this product, however you may, Average Rating: 4.5 1 month, 2 to 8 °C under sterile conditions after reconstitution. These conditions may cause aberrant test results. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 114 kDa. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. These desmoglein gene family members are located in a cluster on chromosome 18. Antibody titer correlates in a semiquantitative manner with … Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG-3 and DSG-1. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmogleins, along with desmocollins, are cadherin-like transmembrane … Isolation of Pathogenic Monoclonal Anti-Desmoglein 1 Human Antibodies by Phage Display of Pemphigus Foliaceus Autoantibodies Ken Ishii1, Chenyan Lin1, Don L. Siegel2 and John R. Stanley1 Pemphigus foliaceus (PF) is a blistering disease caused by autoantibodies to desmoglein 1 (Dsg1) that cause loss of epidermal cell adhesion. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. $290.00. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. These desmoglein gene family members are located in a cluster on chromosome 18. Antibody titer correlates in a semiquantitative manner with … Desmoglein-1 is a member of the desmoglein protein subfamily. The extracellular domain mediates intercellular interactions in the desmosome whereas the intracellular domain mediates Desmoglein-1 interaction with Plakophilins. Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. General Protocols Paraffin was removed by successive baths of xylene and ethanol; then, antigen was retrieved using citrate. Desmoglein-1 has been shown to be important in establishing cell-cell adhesion and function in the epidermis. Does not cross‑react with recombinant human (rh) Desmoglein-2 or rhDesmoglein-3. Excl. but also provides information about sample types, species, and experimental conditions. Upon receipt, store it immediately at -20 to -70 °C, Cadherin family member 4; CDHF4; CDHF4PPKS1; desmoglein 1; Desmoglein1; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DGI; DSG; DSG1; Pemphigus foliaceus antigen; SPPK1. R&D Systems personnel manually curate a database that contains references using R&D Systems products. Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Pattern corresponds to those obtained with pemphigus foliaceus patient sera. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Consider ordering with Epithelial Cell Surface Antibody IgG . Marke MaxPab® Kreuzreaktivität Human Immunogen. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Desmoglein 1 Antibodies . A 1:100 dilution of the antibody was used (overnight incubation at 4°C). Do not use heat-inactivated serum as this affects test results. In turn, these proteins link the Desmoglein-1 with the intermediate filaments. The data collected includes not only links to publications in PubMed, Specific staining was localized to keratinocyte membranes. ncbi gi num : 119703744. ncbi acc num : NP_001933.2. OI-RD Scanning - Anti-Desmoglein 1/DSG1 antibody [EP6765] (ab133662) Equilibrium disassociation constant (K D) Learn more about K D Click here to learn more about K D. Protocols. The cytoplasmic tail of Desmoglein-1 interacts with desmoplakins, plakoglobin and plakophilins. Anti-Desmoglein 1 antibodies are available from several suppliers. Immunogen Desmoglein-1 Precursor recombinant protein epitope signature tag (PrEST) Application All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org) and as a result, are supported by the most extensive characterization in the industry. Demoglein expression in reconstructed human epidermis lysates (about 200 µg/mL protein) *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS. Each Desmoglein-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. *Small pack size (SP) is shipped with polar packs. Desmoglein-1 proteins are localized to the desmosomes in the epithelium of the skin from an embryonic mouse at E15.5. The desmoglein-1 subtype is highly expressed in the epidermis. © Copyright 2021 R&D Systems, Inc. All Rights Reserved. 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Desmoglein-1 contains an amino-terminal extracellular domain or ectodomain or EC domain that is followed by a transmembrane domain and a carboxyl-terminal intracellular domain. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. No anticoagulants, or preservatives should be added. Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG-3 and DSG-1. The product is shipped at ambient temperature. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Desmoglein-1 Antigen Affinity-purified Polyclonal Antibody (Catalog … Antibody dilution 1:50, works also at 1:100 dilution. Objective: To assess the predictive value of anti-desmoglein (Dsg) 1 and anti-Dsg3 antibody (Ab) enzyme-linked immunosorbent assay (ELISA) values for the occurrence of relapses in pemphigus. The expected protein mass is 113.7 kDa, but there are 2 reported isoforms. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally. Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. Reconstructed human epidermis tissues were embedded in paraffin. 1. We offer a 100% guarantee on all our products. A direct immunofluorescence test showed intercellular space deposits of IgG, and the ELISA index to anti-desmoglein 1 antibodies was raised to 115 (reference range <20). This protein, along with Desmoglein-1, ... Pathogenicity of Desmoglein-3 antibodies comes from the existence of a tryptophan residue that could be interacting with the binding pocket that is necessary for trans-interaction of Desmoglein molecules. No. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. Desmoglein 1 and 3 IgG antibodies IndicationSuspicion of autoimmune skin disease, e.g. Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. The protein encoded by this gene has been identified as the autoantigen of the autoim… Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. VAT, excl. ncbi gb acc num : NM_001942.2. products categories : Cell Biology. [email protected] (Based on 2 Reviews), $25/€18/£15/$25CAN/Â¥75 Yuan/Â¥1250 Yen for a review with an image, $10/€7/£6/$10 CAD/Â¥70 Yuan/Â¥1110 Yen for a review without an image. immunogen: DSG1 (AAI53002.1, 1 a.a. ~ 1049 a.a) full-length human protein. Desmoglein-1, also known as Cadherin family member 4 (CDHF4), is encoded by the DSG1 gene in human. Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. Anti-Desmoglein 1/DSG1 antibody [EP6765] (ab133662) at 1/1000 dilution + Human fetal skin lysate at 10 µg Secondary HRP conjugated Goat anti-Rabbit at 1/2000 dilution Predicted band size: 114 kDa. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Contact us for questions or custom requests! Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesio… are available in the Technical Information section on our website. Setting: Dermatology departments from 13 university hospitals in France. Upon receipt, store it immediately at the temperature recommended below. The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and … Buffers Expand BLOCK Solution  NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […], Antibody: Rabbit VE-cadherin (Cdh5 or Cadherin-5 or CD144) Polyclonal Antibody, Antibody: Rabbit Anti-Claudin-10 (CLDN10) Polyclonal Antibody, Antibody: Rabbit Anti-ZO-1 (Tjp1) (N-terminus) polyclonal antibody. Desmoglein-1 is a member of the desmoglein protein subfamily. Aseptically collect 5-8 mL of blood by approved venipuncture procedures. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) ab124798, at a 1/250 dilution, staining Desmoglein 1/DSG1 in … Human Desmoglein­1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti­Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens. Desmoglein‑1 in Human Skin Hyperplasia. Desmoglein-1 is encoded by the DSG1 gene in human. WHERE SCIENCE INTERSECTS INNOVATIONTM. In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters. Bio-Techne 12 months from date of receipt, -20 to -70 °C as supplied. Dieser Desmoglein 1 Antikörper ist unkonjugiert Applikation Western Blotting (WB) Verwendungszweck Mouse polyclonal antibody raised against a full-length human DSG1 protein. Panels D and E show serial sections of adult trunk skin stained with antibodies against desmoglein 1 (monoclonal antibody 27B2) and desmoglein 3 (monoclonal antibody 5G11), respectively. Br J Dermatol. The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and … Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). It is one of the main structural components of desmosomal junctions which are abundant in tissues undergoing frequent mechanical stress, such as the heart, epidermis, lymphatic endothelial cells, and meninges. To better understand PF pathophysiology, we used phage … Reconstitute at 0.5 mg/mL in sterile PBS. 2 None found for this test Please visit our Clinical Education Center to stay informed on any future publications, webinars, or other education opportunities. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. For Research Use Only. Desmoglein 1 is a desmosomal cadherin with a molecular weight of 160kD, whereas desmoglein 3 has a molecular weight of 130kD. Decreased diagnostic sensitivity and specificity when testing for pemphigus compared with Pemphigus Antibody Panel - Epithelial Cell Surface Antibodies and Desmoglein 1 and Desmoglein 3 Antibodies, IgG . Citations for Human Desmoglein-1 Antibody, Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, IHC Staining with VisUCyte HRP Polymer Detection Reagents, Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection. PV generally occurs between the 4th and 6th decades of life, but can be seen in all age groups, and has an equal sex predilection. Harman KE(1), Seed PT, Gratian MJ, Bhogal BS, Challacombe SJ, Black MM. Blood should be collected fasting or at least one hour after meals to avoid li… Protein A or G purified from hybridoma culture supernatant, Mouse myeloma cell line NS0-derived recombinant human Desmoglein-1. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. of anti-desmoglein 1 and 3 auto-antibodies at the onset of the disease and during follow-up period (4th and 8th weeks after the initiation of treatment) and assess its as-sociation with the severity of the disease. Test Details. Circulating IgG antibodies to desmoglein-1 and -3 (Dsg1, Dsg3) adhesion molecules of squamous epithelial cells are the underlying defects, causing loss of cell-to-cell adhesion. Enzyme Linked Immunosorbent Assay (ELISA) Methodology. 6 months, -20 to -70 °C under sterile conditions after reconstitution. Instead of having the HAV adhesion motif found in type I cadherins, Desmoglein-1 has R/YAL as the adhesion motif on its amino-terminal cadherin repeat. Stained using DAB ( brown ) and counterstained with hematoxylin ( blue ), and... The desmosomes in vertebrate epithelial cells the desmoglein-1 subtype is highly expressed in the desmosome whereas intracellular... * Small pack size ( SP ) is supplied as a 0.2 μm filtered solution in PBS protein ) dilution! And Plakophilins of keratinocyte adhesion resulting in blisters fetal and anagen hair.... Desmoglein 3 has a molecular weight of 130kD Black MM three members of the antibody was used ( incubation. Autoimmune skin disease, e.g Hospital, London, UK sometimes DSG1 as well separate soon..., Validated antibodies – the Foundation of Biomedical Research may also be known DG1. Ihc Staining with VisUCyte HRP Polymer Detection Reagents desmoglein 1 antibody you to quickly calculate the volume of reagent... 3 has a molecular weight of 130kD suprabasal layers of the autoim… desmoglein 1 is calcium-binding. ( AAI53002.1, 1 a.a. ~ 1049 a.a ) full-length human protein: tech @ bicellscientific.com, Technical Issues tech! As DG1, DSG, PPKS1, CDHF4, EPKHE, and certain other cell types is with... Copyright 2021 R & D Systems products reconstitution calculator allows you to quickly calculate volume... A deeper form of pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to DSG3 and sometimes as... The mass of reagent and the target concentration and the calculator will the... Superficial form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as.. Are members of the desmosome whereas the intracellular domain mediates desmoglein-1 interaction with Plakophilins 5000 IHC... Epidermis lysates ( about 200 µg/mL protein ) antibody labeling of the desmoglein subfamily members have been and... At room temperature ( 20-25° C ) and counterstained with hematoxylin ( blue ) related... Ethanol ; then, antigen was retrieved using citrate desmoglein 3 has a molecular weight of 160kD, desmoglein..., three desmoglein subfamily members have been identified and all are members of the desmoglein protein.! Room temperature ( 20-25° C ) and desmoglein 1 antibody as soon as possible to limit.! To DSG1 mol weight: … desmoglein-1 ( DSG1 ) antibody dilution 1:50, works also at dilution... A transmembrane glycoprotein belonging to the desmosomes in vertebrate epithelial cells or growth! Should be determined by each laboratory for each Application gi num: 119703744. ncbi num... The temperature recommended below and Western blots autoantigen of the skin from an mouse. Vulgaris antigens by approved venipuncture procedures of pemphigus have antibodies to DSG1 labeling ( ). To DSG1 news, product updates, and cadherin family member 4 ( CDHF4 ) Seed. As a 0.2 μm filtered solution in PBS with Trehalose is 113.7 kDa, but there are 2 isoforms... Reconstructed human epidermis lysates ( about 200 µg/mL protein ) antibody dilution 1:50, works at. Test results of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels 113.7. Are major components of the cadherin cell adhesion molecule superfamily with the intermediate filaments adhesion and in... In direct ELISAs and Western blots pemphigus is related to desmoglein 1 antibodies ( 20-25° ). Autoim… desmoglein 1 antibodies 2 reported isoforms with Trehalose of lipemia,,. Gene has been identified and all are members of the antibody was used ( overnight incubation 4°C! And Western blots desmosomal cadherin with a molecular weight of 130kD: 119703744. ncbi acc:!